Journal: Methods in molecular biology (Clifton, N.J.)
Article Title: Assessment of Glial Function in the In Vivo Retina
doi: 10.1007/978-1-61779-452-0_33
Figure Lengend Snippet: Confocal images of retinal vessels. (a) Retinal arterioles (a), venules (v), and capillaries are filled with dextran fluorescein isothiocyanate and imaged with confocal microscopy. The optic disc is at the lower left. The luminal diameter of the upper arteriole is measured with confocal line scans (black line). The nearby white bar indicates the location of the flickering light stimulus. Scale bar, 250 μm. (b) Line scan image obtained from the arteriole in (a). Distance (across the black line in (a)) is plotted as a function of time. A flickering light (white bar in (b)) evokes vessel dilation, indicated by the widening of the vessel cross section. The uneven edges of the vessel are caused by a respiratory movement artifact. Modified from ref. (12).
Article Snippet: Blood Vessel Labeling Dextran fluorescein isothiocyanate, 2,000,000 MW (Sigma) dissolved in saline (3% solution).
Techniques: Confocal Microscopy, Modification